Journal: Translational Cancer Research
Article Title: Obovatol induces apoptosis in breast cancer by downregulating the PI3K/Akt pathway
doi: 10.21037/tcr-2025-1-2627
Figure Lengend Snippet: Ob reduced the development of MDA-MB-231 cells in vivo . (A) The timetable for Ob-treated mice with xenograft tumors originating from MDA-MB-231 cells. (B-D) Effects of Ob treatment on tumor volume and weight. (E) Effects of Ob treatment on extending survival duration in mice. (F) Effects of Ob treatment on the body weight index of mice. (G) IHC assays were used to quantify the expression of PCNA, Ki67, Bcl-2, and Bax in tumor tissues. (H) Typical histological pictures of tumor slices stained with H&E. The tumor volume and body weight of the mice were checked every two days. *, P<0.05; **, P<0.01; ***, P<0.001 ( vs. control group). ADR, doxorubicin hydrochloride; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; Con, control; H&E, hematoxylin and eosin; IHC, immunohistochemistry; Ob, obovatol; PCNA, proliferating cell nuclear antigen.
Article Snippet: The following primary antibodies were used: proliferating cell nuclear antigen (PCNA; bs-2007R), Ki67 (bs-52455R), Bax (bs-52316R), and rabbit secondary antibody (bs-0295G-HRP) were obtained from Bioss (Beijing, China).
Techniques: In Vivo, Expressing, Staining, Control, Immunohistochemistry